Coding
Part:BBa_K2586003:Design
Designed by: Robert Warneke, Janek Meißner Group: iGEM18_Goettingen (2018-09-10)
Design Notes
In order to avoid the PstI restriction site, the Adenin at position 1135 was changed to a Thymin.
Source
This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.
References
(Malone et. al. 2015): EPSPS gene amplification in glyphosateâ€resistant Bromus diandrus
Subtiwiki: [http://subtiwiki.uni-goettingen.de/v3/gene/view/CA00DFB480CBDC8AC31559958EF8E97E1E9BD4E1 (aroE)]